Search results for " yeast"
Article
Fermentation for the Future
Jan 01, 2015
By Cynthia Challener, PhD
Microorganisms—bacteria and yeast—have been widely used for the production of genetically engineered (recombinant) biopharmaceuticals. Typical examples…
Article
Antibody Production in Microbial Hosts
Challenges associated with the production of antibodies in microbial hosts
Microbial cells such as yeast and bacteria possess many advantages, such as fast growth, well-known genetics, and low…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
In addition to high cell density mammalian expression, expanded utilization of other expression systems such as microbial and yeast will support these newer biotherapeutics (BioTx). The diversity of B…
Article
Evolution of the Monoclonal Antibody Purification Platform
Monoclonal antibodies expressed in yeast
While mammalian cells (e.g., such as Chinese hamster ovary [CHO]), have been the primary expression system for mAb products; other expression systems such …
Article
A Closer Look at Affinity Ligands
…bilized 14 kD single variable domain of heavy chain (VHH) antibody that is recombinantly produced in yeast. The single-domain heavy chain antibody fragments from Camelidae have also been utilized suc…
Article
Paring Down Impurities in Downstream Processing
Biopharmaceutical expression systems range from yeast and insects to mammals and plants. Bioreactors to house these systems might be stirred-tank, air-lift, fixed-bed, hollow-fiber perfusion, or suspe…
Article
Recent Advances in the Use of Exoglycosidases to Improve Structural Profiling of N-glycans from Biologic Drugs
First, recombinant production of all enzymes was achieved in Escherichia coli or yeast hosts. This required discovery of some genes encoding plant enzymes that had never been produced recombinantly (e…
Article
The Challenge of Disruptive Technologies in Bioprocessing
… of the ever-increasing biomass from highly productive mammalian cell culture, and even more so with yeast systems,” notes Jagschies.
The high cost, or perceived cost, of an established techno…
Article
Sanitization of ÄKTA™ pilot 600 Using Sodium Hydroxide
In a microbial challenge test, parts were exposed to a bacterial strain recommended by the United States Pharmacopoeia (USP 38) and to yeast strains commonly used in production environments. Sanitizat…
Article
Platform Technologies Improve Protein Expression
Tools enabling modification of metabolic pathways for glycosylation in algae and yeast glycosylation are also noteworthy. “These approaches offer avenues for designing better fit-for-purpose molecules…